Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.194530 |
Chromosome: | chromosome 4 |
Location: | 2277488 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre04.g220300 | (1 of 6) IPR001680//IPR015943//IPR017986//IPR020472 - WD40 repeat // WD40/YVTN repeat-like-containing domain // WD40-repeat-containing domain // G-protein beta WD-40 repeat | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATGATGCGCTCACCTGTGTAGCTAGGCTC |
Internal bar code: | TTAGGTCAGGACCCGTCAGGTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 602 |
LEAP-Seq percent confirming: | 94.5083 |
LEAP-Seq n confirming: | 740 |
LEAP-Seq n nonconfirming: | 43 |
LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CACACACTCATACGCATCCC |
Suggested primer 2: | ACGAAAGGATGTACCGAACG |