| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.194550 |
| Chromosome: | chromosome 4 |
| Location: | 508643 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217934 | (1 of 1) K07101 - uncharacterized protein (K07101) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTGTAACGTCTTTTCTGGCAGAGCTGCC |
| Internal bar code: | TGCGGATGTCGAGCGACCGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 921 |
| LEAP-Seq percent confirming: | 89.9809 |
| LEAP-Seq n confirming: | 9906 |
| LEAP-Seq n nonconfirming: | 1103 |
| LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGATGAGCTGTGTAGACGA |
| Suggested primer 2: | AACTGTGCATGGGTGTGAAA |