| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.194564 |
| Chromosome: | chromosome 12 |
| Location: | 4386570 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g520550 | TRN1 | (1 of 1) K18752 - transportin-1 (TNPO1); Importin beta | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCATCTGGCGGAGGATTCCACGCAGAGA |
| Internal bar code: | GTCAGGTTGGAGTCCTAGATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 223 |
| LEAP-Seq percent confirming: | 99.7006 |
| LEAP-Seq n confirming: | 1332 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACAAGAGCGGAAGTTCGAC |
| Suggested primer 2: | CGTGGAGACCGACTACACCT |