| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.194622 |
| Chromosome: | chromosome 8 |
| Location: | 2420509 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g371301 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGAAGCTCCATTCAACAACGCTGGACAT |
| Internal bar code: | CCACAATAGCCAGTGGTCGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 453 |
| LEAP-Seq percent confirming: | 73.919 |
| LEAP-Seq n confirming: | 4342 |
| LEAP-Seq n nonconfirming: | 1532 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGGTTGTGCATTCGTGAT |
| Suggested primer 2: | TGTCCCTGGGTTCTACTTGG |