| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.194630 |
| Chromosome: | chromosome 3 |
| Location: | 8362823 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g198750 | (1 of 2) K02327 - DNA polymerase delta subunit 1 (POLD1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAATGCTAGACTGCCTATGCGTTCTGATA |
| Internal bar code: | TCGCTCGTCCGCATGGGTCCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 278 |
| LEAP-Seq percent confirming: | 98.2318 |
| LEAP-Seq n confirming: | 500 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTGGTTGGTGAGTTCCCT |
| Suggested primer 2: | ACACACACGCACACACACAC |