| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.194640 |
| Chromosome: | chromosome 3 |
| Location: | 5250749 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g183150 | (1 of 1) 3.4.24.21 - Astacin / Crayfish small-molecule proteinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCCTACTGGGCCCAAATCGGCGAGTGCA |
| Internal bar code: | AGCCCCGAGGGCGGGGAGGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1067 |
| LEAP-Seq percent confirming: | 99.5754 |
| LEAP-Seq n confirming: | 4221 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACCGCAACACCACATACTG |
| Suggested primer 2: | AACGAAAACTCGTCACCCAC |