| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.194655 |
| Chromosome: | chromosome 16 |
| Location: | 259749 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g694402 | (1 of 54) IPR001680//IPR017986 - WD40 repeat // WD40-repeat-containing domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTCCTCAACCTCACTGGTCCTCAACCTCA |
| Internal bar code: | GTCGCAGCAATAACTTTCACTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 132 |
| LEAP-Seq percent confirming: | 99.7195 |
| LEAP-Seq n confirming: | 711 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGCATGTACCGGAGTCAC |
| Suggested primer 2: | TAACAGCCCAGGTCCTCACT |