| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.194660 |
| Chromosome: | chromosome 16 |
| Location: | 26878 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g696050 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTAGGGGCGGTTGGACTGCAAGGGGAAGG |
| Internal bar code: | CGCGGCGTGCTCCAATCCACAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 627 |
| LEAP-Seq percent confirming: | 99.8542 |
| LEAP-Seq n confirming: | 2054 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCAAGGGAAACCACTACG |
| Suggested primer 2: | TACTTGCTCACAATGCAGGC |