Insertion junction: LMJ.RY0402.194665_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.194665
Chromosome:chromosome 7
Location:1694569
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g325650 (1 of 3) PF10601 - LITAF-like zinc ribbon domain (zf-LITAF-like) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCGTTTCCTTGCATCCTGCGCAAAGATGG
Internal bar code:GTATTCACAGATTATCTCAGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:746
LEAP-Seq percent confirming:93.6599
LEAP-Seq n confirming:23976
LEAP-Seq n nonconfirming:1623
LEAP-Seq n unique pos:46

Suggested primers for confirmation by PCR

Suggested primer 1:ACGAGATGAGGGGTGTTACG
Suggested primer 2:TTGTCGCTGACAGTTTCGAC