| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.194669 |
| Chromosome: | chromosome 13 |
| Location: | 2379958 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g579450 | CST1 | Chlamydomonas-specific membrane transporter of unknown function | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAAGTCAAATTGCATGCTCAAACCATGTT |
| Internal bar code: | CGCTCAGGGCTCCGCATGAGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 698 |
| LEAP-Seq percent confirming: | 99.7807 |
| LEAP-Seq n confirming: | 910 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATCAGGAAGCAGTTGACG |
| Suggested primer 2: | TGACCTCGCTGTTGACTTTG |