Insertion junction: LMJ.RY0402.194681_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.194681
Chromosome:chromosome 1
Location:4602885
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g031600 (1 of 2) PF05915 - Eukaryotic protein of unknown function (DUF872) (DUF872) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCACGCCACCTGGCACCAGCTCGCCACCAC
Internal bar code:AACGTATACACCGCGAGATCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:68
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:134
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GTGCCGACGACCTTTTACAT
Suggested primer 2:AACCAAACGCAACACTTTCC