| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.194693 |
| Chromosome: | chromosome 9 |
| Location: | 6654272 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g408750 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGATGGTTGCTGTGCCTTTCAGCGACAGGT |
| Internal bar code: | ATCTATAGTCTACCGGAGCTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 954 |
| LEAP-Seq percent confirming: | 99.6772 |
| LEAP-Seq n confirming: | 3088 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTCCTTTGCATTGGGTTTG |
| Suggested primer 2: | CGGTGTGACCAATGTTTCAG |