| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.194729 |
| Chromosome: | chromosome 5 |
| Location: | 1108370 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g245900 | BCA2 | (1 of 1) PTHR11825//PTHR11825:SF46 - SUBGROUP IIII AMINOTRANSFERASE // BRANCHED-CHAIN-AMINO-ACID AMINOTRANSFERASE 3, CHLOROPLASTIC; Branched-chain amino acid aminotransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCTACCCTCGAGGCTCCGCGACCCCCCC |
| Internal bar code: | TGCCTGTCGTCGCAGACGGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGAGTAGCAGAAGGCAGGG |
| Suggested primer 2: | CTACTGCTGCTACTGCTGCG |