| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.194731 |
| Chromosome: | chromosome 8 |
| Location: | 693670 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g360250 | DUR31,DUR4,DUR3B | Urea active transporter; (1 of 3) PTHR11819:SF94 - SODIUM-DEPENDENT MULTIVITAMIN TRANSPORTER-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTACATCACTACACCGGCAGGAATGAGGA |
| Internal bar code: | ATGCAACCTTTGTTTTCGTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 405 |
| LEAP-Seq percent confirming: | 93.5743 |
| LEAP-Seq n confirming: | 233 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCGCTCTCATTCCTTAGAC |
| Suggested primer 2: | CCAACTCAGCGACACGTTTA |