Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.194741 |
Chromosome: | chromosome_13 |
Location: | 238371 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre13.g563700 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GAATCTTTGGGCGGATAGTAGTTGCGGGTC |
Internal bar code: | CGTGAGAGGACGTAGCGCTGAGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 83 |
LEAP-Seq percent confirming: | 98.8539 |
LEAP-Seq n confirming: | 1725 |
LEAP-Seq n nonconfirming: | 20 |
LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCTTGTATACCCGCTCCTCA |
Suggested primer 2: | TGAAACGGGATGTGATGTTG |