| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.194784 |
| Chromosome: | chromosome 3 |
| Location: | 6568223 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g195900 | (1 of 7) PTHR11584//PTHR11584:SF381 - SERINE/THREONINE PROTEIN KINASE // PROTEIN PQN-80, ISOFORM B | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAGAATAACGCAGAAGCGGAACCTTTGCG |
| Internal bar code: | GTGGTCGATTATAGTAAGGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 23 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 108 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATATACCTCGACGGACGC |
| Suggested primer 2: | TGACGAGCACTATCACTCCG |