Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.194830 |
Chromosome: | chromosome 13 |
Location: | 3282122 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
intergenic |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCGTGTGTGGTGCTGTCGCCTGGCAGCA |
Internal bar code: | TTCAGGCTGTGAAAGCGCCCAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 690 |
LEAP-Seq percent confirming: | 98.0239 |
LEAP-Seq n confirming: | 3770 |
LEAP-Seq n nonconfirming: | 76 |
LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGCACATGGTGGATACTTTG |
Suggested primer 2: | CCGAATGGCCTTACTCCATA |