| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.194864 |
| Chromosome: | chromosome 16 |
| Location: | 1029603 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g649250 | (1 of 4) PF13911 - AhpC/TSA antioxidant enzyme (AhpC-TSA_2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGCGTGTGGCGGCCCAAGGTGCGGGGGC |
| Internal bar code: | ATGTCTTGTATCCGGAATTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 901 |
| LEAP-Seq percent confirming: | 63.2955 |
| LEAP-Seq n confirming: | 557 |
| LEAP-Seq n nonconfirming: | 323 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGGAGGTTGTTTGAGTCAT |
| Suggested primer 2: | TTCCTACTCCCACTCATGGC |