| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.194879 |
| Chromosome: | chromosome 12 |
| Location: | 1141281 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g490200 | (1 of 1) PTHR24362//PTHR24362:SF160 - SERINE/THREONINE-PROTEIN KINASE NEK // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGAGGGCTGTGCCCCGAGGTTATGATGGG |
| Internal bar code: | GACTCGATTAACATCTAAGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 466 |
| LEAP-Seq percent confirming: | 99.824 |
| LEAP-Seq n confirming: | 3404 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGTTTAACGCGTGGTGCAG |
| Suggested primer 2: | GATTATGGTGGCTTGCAGGT |