| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.194888 |
| Chromosome: | chromosome 12 |
| Location: | 525851 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g493000 | (1 of 1) IPR006153//IPR020846 - Cation/H+ exchanger // Major facilitator superfamily domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGAGGAGGAGGGGGAGGAAGCGGCCTGGA |
| Internal bar code: | GTCCGAGTTCTCCAGGGGCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 806 |
| LEAP-Seq percent confirming: | 98.6229 |
| LEAP-Seq n confirming: | 9525 |
| LEAP-Seq n nonconfirming: | 133 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGTTTGTGATCAGGGGTT |
| Suggested primer 2: | CATGATGAGTCCAATGCTGG |