| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.194909 |
| Chromosome: | chromosome 8 |
| Location: | 4561531 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g382850 | LYR2,ISD11 | Complex 1 protein (LYR family) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGGATGAAGGGGGGCATGGGCCGCGGCA |
| Internal bar code: | GTGCATCATTCCTGGACACGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 771 |
| LEAP-Seq percent confirming: | 99.2415 |
| LEAP-Seq n confirming: | 6542 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAAGCTTAGAGAGAGGCGG |
| Suggested primer 2: | ACAAAACCCATGCCTACTCG |