| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.194918 |
| Chromosome: | chromosome 8 |
| Location: | 1807300 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g366500 | intron|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCATGAATAGCGTGTCAAAGAAACAGGTG |
| Internal bar code: | GAGGCGAGGTGAGTCAGCGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 136 |
| LEAP-Seq percent confirming: | 84.4444 |
| LEAP-Seq n confirming: | 114 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACACACGCGGCTTATTACC |
| Suggested primer 2: | ATTCGATGGATTCGACTTGC |