| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.194918 |
| Chromosome: | chromosome 9 |
| Location: | 6900089 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g410250 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTGCTAGCATCATGTATGCGGAACAGGG |
| Internal bar code: | TCGTAAGTCAAGCCATTAATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 937 |
| LEAP-Seq percent confirming: | 99.7433 |
| LEAP-Seq n confirming: | 4274 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGGGCTGACAAAAGACTC |
| Suggested primer 2: | AGCTTCTTCAGCAGCTCGTC |