Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.194975 |
Chromosome: | chromosome_16 |
Location: | 3525355 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre16.g677382 | sense | 5'UTR|outside_mRNA |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CACAAGGAGGCTCCATTGCTCTCGGCACTG |
Internal bar code: | GGTACCGGGATACCGAACACAT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 906 |
LEAP-Seq percent confirming: | 99.7197 |
LEAP-Seq n confirming: | 1779 |
LEAP-Seq n nonconfirming: | 5 |
LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTCTACAGCTGAAGGGGCAG |
Suggested primer 2: | GCACATAGCTACAGGCACGA |