Insertion junction: LMJ.RY0402.194987_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.194987
Chromosome:chromosome 6
Location:5810413
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g287600 (1 of 7) PF12796//PF13637 - Ankyrin repeats (3 copies) (Ank_2) // Ankyrin repeats (many copies) (Ank_4) 5'UTR_intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTTCGACGCCGCTGCGAGTCCCCGAAGAG
Internal bar code:AACATTTTCTGGCGCAGACGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:776
LEAP-Seq percent confirming:99.4209
LEAP-Seq n confirming:515
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GTGCAATCAGGGACGGTAGT
Suggested primer 2:CCACCTTTTCGTGAGTCGAT