| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.195005 |
| Chromosome: | chromosome 9 |
| Location: | 4683612 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396846 | (1 of 2) PTHR22957//PTHR22957:SF148 - TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN // RE26521P | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCATGGCTACTTCTGTATAATATGCTTCA |
| Internal bar code: | TGTCTGCTCGATGACGGGCTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 0 |
| LEAP-Seq percent confirming: | 99.7416 |
| LEAP-Seq n confirming: | 772 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTAACAGCTGCCTGGTCA |
| Suggested primer 2: | CCGGAGGCAACAAAATAGAA |