| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.195015 |
| Chromosome: | chromosome 6 |
| Location: | 6464802 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g292850 | CDC6 | (1 of 2) PTHR10763 - CELL DIVISION CONTROL PROTEIN 6-RELATED; Pre-initiation complex, subunit CDC6 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTGCAAGCGCGGATTGACAAAAACAAAA |
| Internal bar code: | CTCCCCGGCCCGGGAGCGTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 12 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGGAGGAAAGGAAATGAA |
| Suggested primer 2: | GTGTTGCCATTTGTCATTGC |