| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.195044 |
| Chromosome: | chromosome 11 |
| Location: | 2112200 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g469300 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGAGCCCGAGCCGGCAAGGCCTGACTCCC |
| Internal bar code: | GATGTACAATATCGTGGGTTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 668 |
| LEAP-Seq percent confirming: | 98.5437 |
| LEAP-Seq n confirming: | 1218 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTACAGCATTTGTGCTGCC |
| Suggested primer 2: | CCTTCAGCTTGCCAATTTTC |