Insertion junction: LMJ.RY0402.195107_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.195107
Chromosome:chromosome 14
Location:2201877
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre14.g622750 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTCTTGCATAACAGGCCAGCTCCGGAGCG
Internal bar code:CGTGGACTTGTCACGCGGGTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:765
LEAP-Seq percent confirming:99.8311
LEAP-Seq n confirming:2364
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:CTGTCTCCGCAAAGTCCTTC
Suggested primer 2:GGAGCAACTAGCTTCATGCC