| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.195113 |
| Chromosome: | chromosome 7 |
| Location: | 3718614 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g337700 | PDC1 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTCTCCGACCCACATCGCTGCGACGCTG |
| Internal bar code: | AGGGACCCTACGGCAGTGTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 878 |
| LEAP-Seq percent confirming: | 98.7127 |
| LEAP-Seq n confirming: | 2914 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCGCCGCTATACCATACA |
| Suggested primer 2: | GCGTGGAGTCCCACTAGAAG |