| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.195121 |
| Chromosome: | chromosome 10 |
| Location: | 4203058 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g449950 | (1 of 1) PTHR14593:SF5 - WD REPEAT-CONTAINING PROTEIN 11 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCGCGGAGAAGGTGGCGGGCGCGGCGGC |
| Internal bar code: | CCGATTTGTAGCGGTATGTATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 286 |
| LEAP-Seq percent confirming: | 99.6784 |
| LEAP-Seq n confirming: | 4339 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGCCCAAACTAAACCAAA |
| Suggested primer 2: | TGATCTCACTGAAGAGGGGG |