| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.195130 |
| Chromosome: | chromosome 7 |
| Location: | 2436066 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g328950 | (1 of 1) PTHR12277:SF51 - ALPHA/BETA HYDROLASE FOLD-1 DOMAIN-CONTAINING PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTCATGGCCGCCAGCGCCAGTTTGGCCA |
| Internal bar code: | CGGCCCGTTCCAATACACAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 327 |
| LEAP-Seq percent confirming: | 99.8822 |
| LEAP-Seq n confirming: | 4239 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTTGCCTGCATAACTCTC |
| Suggested primer 2: | CCTGCTCACCGTATCAGGTT |