Insertion junction: LMJ.RY0402.195135_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.195135
Chromosome:chromosome 3
Location:198048
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g144284 CGL110 MATH-domain protein; (1 of 2) K10523 - speckle-type POZ protein (SPOP) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGGCACTCAGAGTCTTCTCCGACCACGCT
Internal bar code:CGTGGCATAGTGGCCCGTGTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:756
LEAP-Seq percent confirming:99.81
LEAP-Seq n confirming:3677
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:GCACGGTACACAGGTGAATG
Suggested primer 2:GTCACAAGTAGCGGGACCAT