| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.195148 |
| Chromosome: | chromosome 8 |
| Location: | 3559808 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g375550 | (1 of 25) IPR013216//IPR029063 - Methyltransferase type 11 // S-adenosyl-L-methionine-dependent methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCAACCGCGTTGCCGCCCTTGGCAGTTA |
| Internal bar code: | GAAGTGATTGGCGTCCCAGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 384 |
| LEAP-Seq percent confirming: | 98.4603 |
| LEAP-Seq n confirming: | 1215 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCTCTTCACCGTCCTCAC |
| Suggested primer 2: | GGGTTTCAACGTAACCTCCA |