| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.195166 |
| Chromosome: | chromosome 1 |
| Location: | 5383738 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g037900 | (1 of 2) K03257 - translation initiation factor 4A (EIF4A) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAATGAGCACGAGCAACACCGGACAGGCC |
| Internal bar code: | GCAGTGTGGCGTGTGTGGTTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 984 |
| LEAP-Seq percent confirming: | 99.0358 |
| LEAP-Seq n confirming: | 5444 |
| LEAP-Seq n nonconfirming: | 53 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTCAGGACCCGCTCTATG |
| Suggested primer 2: | ATGCCATCCTTCACCTCTTG |