Insertion junction: LMJ.RY0402.195198_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.195198
Chromosome:chromosome 13
Location:2742799
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g582600 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGCCATCCACACACACACCACCCAAACCA
Internal bar code:GGCCCTTACCAGCCGCGTCTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:133
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GTAGGTTCCGGGTGTTACGA
Suggested primer 2:CTGACTCTCAAAGGTTCGCC