| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.195213 |
| Chromosome: | chromosome 11 |
| Location: | 244540 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467555 | (1 of 1) PF00023//PF13857 - Ankyrin repeat (Ank) // Ankyrin repeats (many copies) (Ank_5) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCCGACATCGACAAGGTCAACAAGGTGC |
| Internal bar code: | GCTATACTCGGAATGGTTCTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 121 |
| LEAP-Seq percent confirming: | 99.0909 |
| LEAP-Seq n confirming: | 109 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCATAGCAGATCGAAAACG |
| Suggested primer 2: | ATGGAAAAGCGGTACGAGTG |