| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.195222 |
| Chromosome: | chromosome 14 |
| Location: | 2038720 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g621800 | GT90-27,GT90F27 | GT90 family protein 27; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCCGTGGTTGCTGCGGGAAGATTGGGCG |
| Internal bar code: | GGGACGAAGGCGTGATGCAAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 946 |
| LEAP-Seq percent confirming: | 46.2443 |
| LEAP-Seq n confirming: | 7357 |
| LEAP-Seq n nonconfirming: | 8552 |
| LEAP-Seq n unique pos: | 47 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACAACGAGCGGTAGTCAG |
| Suggested primer 2: | CATGTATTGGCAACACTGGC |