Insertion junction: LMJ.RY0402.195227_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.195227
Chromosome:chromosome 1
Location:3733415
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre01.g023950 (1 of 11) PF13229 - Right handed beta helix region (Beta_helix) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGAATCGCCTGCACTGCTCGATGCCTTTG
Internal bar code:GTCTTTAATTTCTGCATTTCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:29
LEAP-Seq percent confirming:99.8687
LEAP-Seq n confirming:1521
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CAGCTAGTTGGGCTAGTGCC
Suggested primer 2:GAGCCGTCTCCTGTAGCACT