| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.195234 |
| Chromosome: | chromosome 17 |
| Location: | 1525580 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g707250 | MFT29 | Major facilitator superfamily transporter; (1 of 82) IPR020846 - Major facilitator superfamily domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTACATCGCGCGCACCTCCATCATGAACTC |
| Internal bar code: | CAGGACTGCTTGATTCAGTATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 257 |
| LEAP-Seq percent confirming: | 99.6802 |
| LEAP-Seq n confirming: | 1870 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTGGGGAAAGATAAGTGC |
| Suggested primer 2: | TCCCTTCCCTTGTAAATCCC |