| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.195267 |
| Chromosome: | chromosome_13 |
| Location: | 2071695 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre13.g577352 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTTCGCGACACTGTTTTCGCCTCCGTACTG |
| Internal bar code: | CGGTTAATCTCTATAGGCCATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 601 |
| LEAP-Seq percent confirming: | 99.3721 |
| LEAP-Seq n confirming: | 11395 |
| LEAP-Seq n nonconfirming: | 72 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCATCCTGCCCTTAGGAGT |
| Suggested primer 2: | CTCGCTGAGAACTGACCACA |