| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.195309 |
| Chromosome: | chromosome 10 |
| Location: | 1794624 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g430950 | RAD54B,SRH18 | (1 of 1) K10875 - DNA repair and recombination protein RAD54 and RAD54-like protein (RAD54L, RAD54); SNF2-related DNA/RNA helicase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGAGCGACGGCGAGGATGAGGACGGCGA |
| Internal bar code: | CAAACGCGGGCCAGAATTATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 21 |
| LEAP-Seq percent confirming: | 66.787 |
| LEAP-Seq n confirming: | 185 |
| LEAP-Seq n nonconfirming: | 92 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGATAGACAGTGTGGCGAG |
| Suggested primer 2: | ACACCTGCAGCTTGTGTACG |