Insertion junction: LMJ.RY0402.195326_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.195326
Chromosome:chromosome_3
Location:8322491
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre03.g199000 PHT1,PHOT,PHOT1 Phototropin antisense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):AGCGAATCAAAACTGTCATGGAGCAGTTTG
Internal bar code:GTGTACGAAGGAGAGGAATAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:750
LEAP-Seq percent confirming:95.4601
LEAP-Seq n confirming:778
LEAP-Seq n nonconfirming:37
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:TGGTCCACAACTTCCAAACA
Suggested primer 2:CTGGTTCAAGGGCATCAACT