Insertion junction: LMJ.RY0402.195333_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.195333
Chromosome:chromosome 6
Location:6087415
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g289800 CAM12 Calmodulin-like protein; (1 of 1) 2.3.1.23//2.3.1.51//2.3.1.67 - 1-acylglycerophosphocholine O-acyltransferase / Lysolecithin acyltransferase // 1-acylglycerol-3-phosphate O-acyltransferase // 1-alkylglycerophosphocholine O-acetyltransferase / Platelet-activating factor-synthesizing enzyme intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCCTTTTCGCTGCGTGACATGAACATAGA
Internal bar code:GGCTGGGTACAATAAGTACTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:837
LEAP-Seq percent confirming:99.8355
LEAP-Seq n confirming:1214
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGCTCTAAGCCTAATGCC
Suggested primer 2:GACCCTCCCAACTCACTGAA