| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.195353 |
| Chromosome: | chromosome 13 |
| Location: | 4533284 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g602700 | SPA1 | Part of COP1/SPA complex E3 ubiquitin ligase complex; (1 of 1) K16240 - protein suppressor of PHYA-105 1 (SPA1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGTAGGTCAGGAGTGCGGAGGAGGAGGAC |
| Internal bar code: | AAATCCCGACTCGGCTTCTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1023 |
| LEAP-Seq percent confirming: | 99.8057 |
| LEAP-Seq n confirming: | 2055 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTGGCTAGCTACGACTTC |
| Suggested primer 2: | ACATCTGCTGAATTACCCCG |