| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.195368 |
| Chromosome: | chromosome 10 |
| Location: | 2848036 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g439700 | CGL28 | conserved expressed putative RNA binding protein; (1 of 89) PF00076 - RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain) (RRM_1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCATTATACCAATACCTTCCGCAACCGTCA |
| Internal bar code: | AAGCCGGCACAGGGCAGGTATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 859 |
| LEAP-Seq percent confirming: | 99.8024 |
| LEAP-Seq n confirming: | 1515 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAACCATTTCTGGTCAGGC |
| Suggested primer 2: | GGGCAATGTAGTTGGCAGTT |