| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.195368 |
| Chromosome: | chromosome 12 |
| Location: | 6482585 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g538400 | RPB4,RPB7B | (1 of 1) K03022 - DNA-directed RNA polymerase III subunit RPC8 (RPC8, POLR3H); DNA-directed RNA polymerase II, 19 kDa polypeptide | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTACAAAGCAGACGTGCCGCTTCAATAGT |
| Internal bar code: | GTATTAACACTTAGTTCAGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 914 |
| LEAP-Seq percent confirming: | 99.8187 |
| LEAP-Seq n confirming: | 1101 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTAGCGCTTCCTGTCCAAC |
| Suggested primer 2: | CTAACCAGGCAGCAGGTCTC |