| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.195400 |
| Chromosome: | chromosome 9 |
| Location: | 3737965 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g391208 | PSRP4 | Plastid-specific ribosomal protein 4 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAAGTTACTACGGTATGCAAAATATCAAC |
| Internal bar code: | GGGCACGCGGTTCGGCACGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 370 |
| LEAP-Seq percent confirming: | 99.6523 |
| LEAP-Seq n confirming: | 2293 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACAGTGGATGTTGAGGGA |
| Suggested primer 2: | GAGAAAACGCTGGTCAAAGC |