| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.195404 |
| Chromosome: | chromosome 14 |
| Location: | 2943454 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g627650 | FAP392 | Flagellar Associated Protein 392 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTAACTAAGAAGGGGGGGTTTGCAAAGAT |
| Internal bar code: | CGCAGTCCTGACCGGAGGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 228 |
| LEAP-Seq percent confirming: | 97.7922 |
| LEAP-Seq n confirming: | 753 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACATCCGTACATGAGCTGG |
| Suggested primer 2: | COULD_NOT_FIND_PRIMER |