| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.195407 |
| Chromosome: | chromosome 2 |
| Location: | 3541183 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095128 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCTAGCCGGCGTAGCCCTGGCCAACCACA |
| Internal bar code: | CCACGGAAGCAGGTGCGTCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 829 |
| LEAP-Seq percent confirming: | 99.854 |
| LEAP-Seq n confirming: | 2052 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATACAGATGACGTGCGGTC |
| Suggested primer 2: | CGGCACAGGACAAGTGTAGA |